Analysis of Rhodamine B in Lipsticks Marketed in Wonosobo, Indonesia, Using Rapid Test Kits and UV–Visible Spectrophotometry

Authors

  • Nova Andina Author
  • Dharmastuti Cahya Fatmarahmi Universitas Muhammadiyah Yogyakarta Author

DOI:

https://doi.org/10.70392/jpns.v3i2.50

Keywords:

Rhodamine B, Lipstick, Rapid Test Kit, Spectrophotometry UV-Vis

Abstract

Rhodamine B is a synthetic dye that is commonly used as a colorant in the paper and textile industries. Rhodamine B is a hazardous dye that is frequently used as a colorant in cosmetics due to its relatively low cost and ability to produce more attractive colors. The use of Rhodamine B in cosmetics can be hazardous to health as it can cause cancer, respiratory tract irritation, eye irritation, skin irritation, and digestive disorders. The objective of this study is to determine the presence of Rhodamine B in lipsticks available on the market in the Wonosobo region. This study employed both qualitative and quantitative methods. The qualitative analysis in this study utilized a rapid test kit to quickly detect the presence of Rhodamine B in lipstick samples based on color changes resulting from the addition of reagents. Samples suspected of containing Rhodamine B were analyzed quantitatively using UV-Vis Spectrophotometry to determine the concentration of Rhodamine B contained in the samples. The results of the qualitative analysis using the rapid test kit showed that, out of 17 lipstick samples tested, 5 samples were suspected of containing Rhodamine B, namely samples 4, 5, 11, 13, and 15. Quantitative analysis using UV–Vis spectrophotometry revealed that the Rhodamine B concentrations were 0.1213 mg/g in sample 4, 0.0861 mg/g in sample 5, 0.0584 mg/g in sample 11, 0.0961 mg/g in sample 13, and 0.1082 mg/g in sample 15. Based on these findings, it can be concluded that several lipsticks suspected of containing hazardous dyes, such as Rhodamine B, are still found in the market and it urges the stakeholder to be more concerned about the dangerous ingredients in cosmetics.

References

Rani, Z., Pulungan, A.F., Ningtias, A., Nasution, H.M. Krim Pelembab Kulit Semangka. LPPM UMNAW. 2023.

Heidi, Putri, D.N., Prayitno, F.A., Damayanti, H.O., Kurniawati, H., Mulya, I.H., Nurjannah, I., Sofiyah, N., Nehru, N.F., Imanta, R.R., Kholishotin, R.N., Marza, T.A. Pola Pemilihan Lipstik di Kalangan Mahasiswi. J. Farm. Komunitas 2018, 5(1), 1–9.

Hidayati, J., Firmawati, N. Prototipe Pendeteksi Rhodamin B Pada Lipstik Menggunakan Sensor TCS3200 untuk Perlindungan Konsumen dari Penggunaan Zat Warna Berbahaya Pada Kosmetik. J. Fis. Unand 2022, 11(2), 235–241. DOI: 10.25077/jfu.11.2.235-241.2022.

Amelia, R., Zairinayati, Z. Analisis Keberadaan Rhodamin B pada Saus Tomat yang Beredar di Pasar Kota Palembang. Ruwa Jurai J. Kesehat. Lingkung. 2020, 14(2), 85–91. DOI: 10.26630/rj.v14i2.2153.

Hipi, D., Malaha, A., Dunggio, T. Analisis Kadar Zat Pewarna Rhodamin B pada Pewarna Bibir yang Beredar di Pasar Minggu Kabupaten Gorontalo. J. Ilm. Adm. Sains 2022, 2(1), 11–21. DOI: 10.47918/jias.v2i1.1074.

Fauziah, S., Komarudin, D., Dewi, C. Identifikasi dan Penetapan Kadar Rhodamin B pada Eye Shadow secara Kromatografi Lapis Tipis dan Spektrofotometri Ultraviolet-Visible. J. Ilm. Kesehat. 2020, 19(2), 81–86.

Yuniarto, P.F., Maryam, N.R. Analisis Kandungan Rhodamin B pada Lipstik yang Beredar di Daerah Kediri. J. Inov. Farm. Indones. 2019, 1(1), 47–59. DOI: 10.30737/jafi.v1i1.626.

Apriliyani, S.A., Martono, Y., Riyanto, C.A., Mutmainah, M., Kusmita, K. Validasi Metode Spektrofotometri UV-Vis untuk Penentuan Kadar Inulin dari Ubi Jalar Kecil (Dioscorea esculenta L.). J. Kim. Sains Apl. 2018, 21(4), 161–165. DOI: 10.14710/jksa.21.4.161-165.

Ramadhan, S.A., Musfiroh, I. Review Artikel: Verifikasi Metode Analisis Obat. Farmaka 2021, 19, 87–92.

Nafiq, U., Yuniarto, P.F., Sulistyowati, Y. Analisis Kandungan Rhodamin B pada Eyeshadow yang Beredar di Daerah Kediri dan Nganjuk. J. Mhs. Kesehat. 2020, 1(2), 131–139.

Laela, D.P. Verifikasi Metode Penetapan Kadar Fenol Total Cara Folin-Ciocalteu Method. 2025, 4(2), 89–99. DOI: 10.25047/plp.v4i2.6193.

Hadriyati, A., Lestari, L., Anggresani, L. Analisis Rhodamin B dalam Bolu Kukus yang Beredar di Kota Jambi dengan Metode Spektrofotometri UV-Vis. J. Farm. Ilmu Kefarm. Indones. 2021, 8(1), 16–21. DOI: 10.20473/jfiki.v8i12021.16-21.

Rohyami, Y., Ratri, H.P.I., Wihyarti, W. Validasi Metode Penentuan Rhodamin B dalam Contoh Saos secara Spektrofotometri UV-Vis dengan Dua Variasi Pelarut. Indones. J. Chem. Anal. 2018, 1(1), 20–28. DOI: 10.20885/ijca.vol1.iss1.art3.

Satiyarti, R.B., Anggaraini, N., Sugiharta, I. Rhodamine B Detection from Inexpensive Blush On in Bandar Lampung City. J. Kartika Kim. 2021, 4(1), 38–41. DOI: 10.26874/jkk.v4i1.66.

Downloads

Published

27-05-2026

Data Availability Statement

The data supporting the findings of this study are available from the corresponding author upon reasonable request.

How to Cite

Andina, N., & Fatmarahmi, D. C. (2026). Analysis of Rhodamine B in Lipsticks Marketed in Wonosobo, Indonesia, Using Rapid Test Kits and UV–Visible Spectrophotometry. Journal of Pharmaceuticals and Natural Sciences, 3(2), 51-59. https://doi.org/10.70392/jpns.v3i2.50

Similar Articles

1-10 of 14

You may also start an advanced similarity search for this article.