In Vitro Free Radical Scavenging Activity of Lagerstroemia speciosa PERS leaves on 1,1-diphenyl-2-picrylhydrazyl

Gita Anna Rezqiyana; Laode Rijai

doi:10.70392/2y5gxp24

Authors

Gita Anna Rezqiyana Fakultas Farmasi Universitas Mulawarman Author
Laode Rijai Fakultas Farmasi Universitas Mulawarman Author

DOI:

https://doi.org/10.70392/2y5gxp24

Keywords:

Antioxidant activity, Free Radical Scavenging Activity, Lagerstroemia speciosa Pers, 1,1-difenil-2-pikrilhidrazil (DPPH)

Abstract

This study aims to determine the yields and free radical scavenging activity based on IC₅₀ value of the extract and the fraction of Lagerstroemia speciosa Pers leaves. The leaves of L. speciosa were macerated using 96% methanol and then practioned using different kinds of solvent based on its polarity including n-hexane, ethyl acetate, and n-butanol. The result showed that the yield of methanol extract obtained from L. speciosa levaes was 5.45% of dried samples obtained, the fraction of n-hexane 19.12%, the fraction of ethyl acetate 4,7%, and the fraction of n-butanol 1.93%. Testing of antioxidant activity is performed with the 1,1-Diphenyl-2- Picryl Hydrazyl (DPPH) by spectrophotometry UV-Visible. Absorbanse measurement was conducted with spectrophotometry at the wave lenght of 516 nm. Data was analyzed with linear regresion to obtain value of IC₅₀. The result of research showed that the extract and fractions of L. speciosa leaves have activity as an antioxidant with the IC₅₀ value of methanolic extract L. speciosa leaves was 12.5 ppm, n-hexane fraction was 18.75 ppm, ethyl acetate fraction was 21.87 ppm, n-butanol was 8.37 ppm.

References

Handayani, S., Kurniawati, I., Rasyif, F.A. Uji Aktivitas antioksidan ekstrak daun karet kebo (Ficus elastica) dengan metode peredaman radikal bebas DPPH (1,1-diphenyl-2-picrlyhydrazil). Jurnal Farmasi Galenika (Galenica Journal of Pharmacy) 2020, 6(1), 141-150.

Sari, I.P., Hidayati, A.R., Muliasari, H. Perbandingan Aktivitas antioksidan infusa simplisia segar dan simplisia kering daun Buni (Antidesma buniues L. Spreng) dengan metode DPPH. Jurnal Sains dan Kesehatan 2023, 5(5), 605-614.

DOI: https://doi.org/10.25026/jsk.v5i5.1792

Asjur, A.V., Santi, E., Musdar, T.A., Saputro, S., Rahman, R.A. Formulasi dan uji aktivitas antioksidan sediaan Face Mist ekstrak etanol kulit apel hijau (Pyrus malus L.) dengan metode DPPH. Jurnal Sains dan Kesehatan 2023, 5(3), 297-305.

DOI: https://doi.org/10.25026/jsk.v5i3.1750

Sies, H., Jones, D.P. Reactive oxygen species (ROS) as pleiotropic physiological signalling agents. Nature Re-views Molecular cell Biology 2020, 21(7), 363-383.

Asih, I.A.R., Setiawan, I.M. Senyawa golongan flavonoid pada ekstrak n-butanol kulit batang Bungur (La-gestroemia speciosa Pers.). Jurnal Kimia (Journal of Chemistry) 2008, 2(2), 12-16.

Rochman, J., Siswoyo, T.A., Ratnadewi, A.I. Antioxidant activity study and α-glucosidase inhibitor phenolic leaf extract Bungur (Lagerstroemia speciosa) from Meru National Park Kediri. Jurnal Ilmu Dasar 2017, 17(1), 39-46.

Wahid, A., Latu, S. Penentuan aktivitas antioksidan ekstrak etanol daun Klabet (Ficus superba Miq.) dengan metode DPPH (1,1-difenil-2-pikrilhidrazil). Jurnal Ilmiah JOPHUS: Journal of Pharmacy UMUS 2023, 4(2), 23-30.

Rosmalena, Widyastuti, P.A., Yazid, F., Ambarwati, N.S.S, Ahmad, I. Phyochemicals and antioxidant activity evaluation of Origanum vulgare (L.) stem bark extracts. Pharmacog J 2021, 13(4), 965-970. DOI: 10.5530/pj.2021.13.124

Ahmad, I., Sulistiarini, R., Ahmad,I. Antioxidant activity of some selected East Borneo plants, Int J Public Health Sci 2015, 4(1), 58-62.

Rosmalena, Putri N.A., Yazid, F., Ambarwati, N.S.S., Omar, H., Ahmad, I. Phyrochemical, in vitro radical scavenging and in vivo oxidative stress analysis of peppermint (Mentha piperita L.). J Adv Pharm Technol Res 2022, 13(2), 133-137. DOI: 10.4103/japtr.japtr_16_22

Wolosiak, R., Druzynska, B., Derewiaka, D., Piecyk, M., Majewska, E., Ciecierska, M., Worobiej, E., Pakosz, P. Verification of Conditions for Determination of anti-oxidant activity by ABTS and DPPH assays – A practical approach. Molecules 2021, 27(1), 50. DOI: https://doi.org/10.3390/molecules27010050

Singh S., Gupta, A., Verma, S. In vitro antioxidant ac-tivities of two medicinal plants on the basis DPPH free radical scavenging activity. Annals of the Romanian Society for Cell Biology 2021, 4807-4811.