Identification of paracetamol compound in traditional herbal medicine for muscle pain relief using thin layer chromatography - Densitometry

Dini Angelina Putri Aulia; Supandi

doi:10.70392/jpns.v2i2.7685

Authors

Dini Angelina Putri Aulia Universitas Muhamadiyah Prof DR HAMKA Author
Supandi Universitas Muhammadiyah Prof DR HAMKA Author

DOI:

https://doi.org/10.70392/jpns.v2i2.7685

Keywords:

Traditional Herbal Medicine for Muscle Pain Relief; , Medicinal Chemicals; , Paracetamol; , Thin Layer Chromatography-Densitometry

Abstract

Traditional medicine consists of substances or formulations derived from plants, animals, minerals, and galenic preparations or mixtures of these materials that have been used for generations as therapy and can be implemented according to the norms applicable in society. The pharmaceutical chemical that is often added to herbal medicine for muscle pain relief is paracetamol. This research aims to prove the presence or absence of the chemical drug paracetamol in the herbal medicine for muscle pain relief found in the Koja District. A total of 10 samples were selected through purposive sampling from herbal stores within the area. The analysis was conducted qualitatively using color reaction test and quantitatively using Thin Layer Chromatography-Densitometry. Color reaction tests indicated that two samples were positive for paracetamol. Analysis using thin-layer chromatography indicated that 3 samples had R_fvalues ranging from 0.3-0.35, close to the standard R_fvalue. Based on the analysis results using Thin Layer Chromatography-Densitometry, the herbal samples that tested positive for containing paracetamol were identified according to the maximum wavelength of the samples that matched the maximum wavelength of paracetamol at 247 nm in samples (S5), (S7), and (S9). The TLC-densitometry method can be used to analyze the presence of paracetamol in traditional herbal medicine for muscle pain relief.

Author Biography

Supandi, Universitas Muhammadiyah Prof DR HAMKA

Fakultas farmasi dan Sains

References

BPOM RI. Public Warning Obat Tradisional, Suplemen Kesehatan, dan Kosmetika Mengandung Bahan Kimia Obat/Bahan Dilarang Tahun 2021. Jakarta, 2021.

Khare, B., Mishra, M.K., Kesharwani, L. Screening of Adulterants in Slimming Herbal Formulation by FTIR Analysis. European Journal of Medicinal Plants 2019, 30(2), 1–8.

BPOM RI. Peraturan Menteri Kesehatan Republik Indonesia Nomor 007 Tahun 2012 Tentang Registrasi Obat Tradisional. 2012, 1–94.

Jayanti, R., Aprilia, H., Lukmayani, Y. Analisis Kualitatif Bahan Kimia Obat (BKO) Glibenklamid dalam Sediaan Jamu Diabetes yang Beredar Dipasaran. Prosiding Penelitian SpeSIA Unisba 2015, 1(2), 649–653.

Susilowati, A., Ramadhan, N.K.K. Perbandingan Aktivitas Diuretik pada Berbagai Produk Seduhan Teh Hijau(Camellia sinensis L.). Majalah Farmasetika 2021, 6, 71–79.

Harimurti, S., Ulandari, S., Widada, H., Damarwati, V.L. Identifikasi Parasetamol dan Asam Mefenamat pada Jamu Pegel Linu dan Asam Urat yang Beredar di Daerah Istimewa Yogyakarta. Journal of Pharmaceutical Science and Clinical Research 2020, 5(2), 179

Kamar, I., Fazrina Z,D.Y., Umairah, R.U. Identifikasi Parasetamol dalam Jamu Pegal Linu Menggunakan Metode Kromatografi Lapis Tipis (KLT). Quimica: Jurnal Kimia Sains dan Terapan 2021, 3(1), 24-29.

Hayun, Karina, M.A. Pengembangan dan Validasi Metode KLT-Densitometri untuk Analisis secara simultan Parasetamol , Asam Mefenamat dan Ibuprofen dalam Jamu “Pegel Linu”. Jurnal Sains Farmasi & Klinis 2016, 2(2), 150–161.

Ningrum, W.A., Wirasti, Sugeng, P. Identifikasi Kandungan Bahan Kimia Obat (Paracetamol Dan Prednison) dalam Kandungan Jamu Rematik GI Dan MT Di Pekalongan. Prosiding The 7th University Research Colloqium STIKES PKU Muhammadiyah Surakarta 2018, 714-720.

Chamidah, S., Yuliastuti, D., Ramadhan, M.F. Identifikasi Dexamethason dalam Jamu Pegal Linu yang Beredar di Cilacap dengan Metode Kromatografi Lapis Tipis (KLT). Jurnal Ilmiah Kefarmasian 2021, 3(1), 39–45

Mosy, F.F., Kuswandani, K. Identifikasi Senyawa Jamu Pegal Linu yang Beredar di Kabupaten Bantul dengan Metode Kromatografi Lapis Tipis. Surya Medika: Jurnal Ilmiah Ilmu Keperawatan dan Ilmu Kesehatan Masyarakat 2019, 14(2), 80.

Sari, B.L., Noviardi, H., Kartini, N.A. Optimasi Waktu Maserasi Parasetamol Dalam Jamu Pegal Linu Yang Beredar Di Bogor Barat. Jurnal Farmamedika 2017, 2(1), 17–29.

Nurhasnawati. H., Rahmayulis, Azmi, D.A. Identifikasi Kandungan Bahan Kimia Obat Parasetamol Pada Jamu Asam Urat Yang Beredar Di Kecamatan Sungai Kunjang Samarinda. Prosiding Seminar Nasional Kimia 2014, 1–5.

Husna, F., & Mita, S. R. (2020). Identifikasi Bahan Kimia Obat dalam Obat Tradisional Stamina Pria dengan Metode Kromatografi Lapis Tipis. Farmaka, 18(2), 16–25.

Kumar, S., Jyotirmayee, K., Sarangi, M. Thin Layer Chromatography: A Tool of Biotechnology For Isolation of Bioactive Compounds from Medicinal Plants. International Journal of Pharmaceutical Sciences Review and Research 2013, 18(1), 126–132.

Pelita, P.D., Wahyuningsih, I., Yuliani, S. Formulasi Solid Snedds (Self-Nano Emulsifying Drug Delivery System) Furosemid Dengan Pengering Aerosil Dan Aktivitas Diuretiknya. Journals of Ners Community 2022, 13(1), 106–118.

Savitri, A., Megantara, S. Metode KLT-Densitometri Sebagai Penetapan Kadar Bahan Aktif Sediaan Farmasi. Farmaka 2019, 17, 455–463.

Ahmad, I., Yanuar, A., Mulia, K., Mun’im, A. Application of ionic liquid based microwave-assisted extraction of the secondary metabolite from Peperomia pellucida (L) Kunth. Pharmacognosy Journal 2017, 9(2), 227-234.

Sylvia, D., Gantina, A., Rusdiana, N. Analisis Sibutramin Hidroklorida pada Jamu Pelangsing Di Kecamatan Curug dengan Spektrofotometri UV. Farmagazine 2018, 5(2), 1–5.