Antibacterial Activity of Lactic Acid Bacteria Isolates Mountain Rice Wash Water (Mayas Rice) Against Propionibacterium acnes
DOI:
https://doi.org/10.70392/jpns.v3i1.42Keywords:
Lactic Acid Bacteria (LAB), Rice water, Propionibacterium acnes, Fermentation, AntibacterialAbstract
This study aims to explore the antibacterial activity potential of fermented dragon fruit peel juice (Hylocereus polyrhizus) fermented using Lactic Acid Bacteria (LAB) isolated from rice washing water (Beras Mayas). The process begins with the preparation of dragon fruit peel samples, which are washed, peeled, and mashed to obtain the juice. The juice is pasteurized and inoculated with a 5% LAB starter, with the addition of a 10% sugar solution before being incubated for 3, 7, 12, and 17 days at 37ºC. pH measurements were taken during fermentation, showing a significant decrease in pH, reaching the lowest value of 3.76 on day 17. The antibacterial activity was measured using the well-diffusion method with the fermented juice at a concentration of 100%, showing a very strong inhibition zone (>20 mm) against Propionibacterium acnes on day 7. The results indicate that the pH changes during fermentation are closely related to the antibacterial activity
References
Yang, Z., Xiaoli, Z., Wen, A., Qin, L. Development of Probiotics Beverage Using Cereal Enzymatic Hydrolysate Fermented With Limosilactobacillus reuter. Food Sci Nutr. 2022, 10(9), 3143-3153. doi:10.1002/fsn3.2913
Sun, J., Zhao, C., Pu, X., Li, T., Shi, X., Wang, B., Cheng, W. Flavor and Functional Analysis of Lactobacillus Plantarum Fermented Apricot Juice. Fermentation 2022, 8(10), 533. doi:10.3390/fermentation8100533
Kim, J., Lee, E., Kim, B., Ham, J., Oh, M. Broad‐spectrum Antimicrobial Activity of Cinnamoyl Esterase‐producing Lac-tobacilli and Their Application in Fermented Rice Bran. J Sci Food Agric. 2021, 102(8), 3130-3139. doi:10.1002/jsfa.11654
O’Neill, A.M., Gallo, R.L. Host-microbiome interactions and recent progress into understanding the biology of acne vul-garis. Microbiome 2018, 6, 177. doi:10.1186/s40168-018-0558-5 PubMed PMID: 30285861.
Coenye, T., Spittaels, K.J., Achermann, Y. The role of biofilm formation in the pathogenesis and antimicrobial susceptibility of Cutibacterium acnes. Biofilm 2022, 4, 100063. doi:10.1016/j.bioflm.2021.100063
Dreno, B., Dekio, I., Baldwin, H., Demessant, A.L., Dagnelie, M.A., Khammari, A., Corvec, S. Acne microbiome: From phyla to phylotypes. Journal of the European Academy of Dermatology and Venereology 2024, 38(4), 657–664. doi:10.1111/jdv.19540.
Cruz, S., Vecerek, N., Elbuluk, N. Targeting Inflammation in Acne: Current Treatments and Future Prospects. Am J Clin Dermatol. 2023, 24(5), 681–694. doi:10.1007/s40257-023-00789-1 PubMed PMID: 37328614.
Mias, C., Mengeaud, V., Bessou-Touya, S., Duplan, H. Recent advances in understanding inflammatory acne: Deciphering the relationship between Cutibacterium acnes and Th17 inflammatory pathway. Journal of the European Academy of Der-matology and Venereology 2023, 37, 3–11. doi:10.1111/jdv.18794.
Niedźwiedzka, A., Micallef, M.P., Biazzo, M., Podrini, C. The Role of the Skin Microbiome in Acne: Challenges and Future Therapeutic Opportunities. International Journal of Molecular Sciences 2024, 25(21), 11422. doi:10.3390/ijms252111422
Carmona-Cruz, S., Orozco-Covarrubias, L., Sáez-de-Ocariz, M. The Human Skin Microbiome in Selected Cutaneous Diseases. Frontiers in Cellular and Infection Microbiology 2022, 12, 834135. doi:10.3389/fcimb.2022.834135
Perdana, C.P., Arifuddin, M., Sastyarina, Y. Pengaruh Waktu Fermentasi Bakteri Asam Laktat Dari Sari Kulit Buah Naga Merah (Hylocereus Polyrhizus) Terhadap Aktivitas Bakteri Propionibacterium acne. Proceeding of Mulawarman Pharma-ceuticals Conferences 2021, 14, 242–8. doi:10.25026/mpc.v14i1.548
Wulandari, A., Arifuddin, M., Sastyarina, Y. Pengaruh Waktu Fermentasi Sari Kulit Buah Naga Merah (Hylocereus poly-rhizus) Terhadap Total Bakteri Asam Laktat (BAL). Proceeding of Mulawarman Pharmaceuticals Conferences 2021, 4, 14–19. doi:10.25026/mpc.v14i1.545
Amanda, R., Rijai, L., Arifuddin, M. Isolation and Identification of Lactic Acid Bacteria from the Washing Water of Mayas Rice, a Traditional Mountain Rice from East Kalimantan. Jurnal Riseta Naturafarm 2025, 2(2), 96–104. doi:10.70392/jrn.v2i2.96104
Hao, P.M., Quoc, L.P.T. Chemical profile and antimicrobial activity of Ocimum gratissimum L. essential oil from Dak Lak province, Vietnam. J Plant Biotechnol. 2024, 51(1), 50–54. doi:10.5010/JPB.2024.51.005.050
Fathoni, M.M., Isnaeni, I., Darmawati, A. Anti-bacterial activity of Rosela Flower Extract (Hibiscus sabdariffa L.) against Extended-Spectrum Beta-Lactamase (ESBL) Eschericia coli. Berkala Ilmiah Kimia Farmasi 2021, 8(1), 7. doi:10.20473/bikfar.v8i1.31204
Dejene, F., Dadi, B.R., Tadesse, D. In Vitro Antagonistic Effect of Lactic Acid Bacteria Isolated From Fermented Beverage and Finfish on Pathogenic and Foodborne Pathogenic Microorganism in Ethiopia. Int J Microbiol. 2021, 2021, 1–10. doi:10.1155/2021/5370556
Manguntungi, B., Saputri, D.S., Mustopa, A.Z., Ekawati, N., Nurfatwa, M., Prastyowati, A., Irawan, S. Antidiabetic, An-tioxidants and Antibacterial Activities of Lactic Acid Bacteria (LAB) From Masin (Fermented Sauce From Sumbawa, West Nusa Tenggara, Indonesia). Annles Bogorienses 2020, 24(1), 27. doi:10.14203/ann.bogor.2020.v24.n1.27-34
Rapoo, S.M., Thaoge-Zwane, M.L. Amylolytic Capability and Performance of Probiotic Strains in a Controlled Sorghum Fermentation System. Fermentation 2024, 10(6), 308. doi:10.3390/fermentation10060308
Zapaśnik, A., Sokołowska, B., Bryła, M. Role of Lactic Acid Bacteria in Food Preservation and Safety. Foods 2022, 11(9), 1283. doi:10.3390/foods11091283
da Silva Vale, A., de Melo Pereira, G.V., de Oliveira, A.C., de Carvalho Neto, D.P., Herrmann, L.W., Karp, S.G., Soccol, V.T, Soccol, C.R. Production, Formulation, and Application of Postbiotics in the Treatment of Skin Conditions. Fermen-tation 2023, 9(3), 264. doi:10.3390/fermentation9030264
Davis, W.W., Stout, T.R. Disc Plate Method of Microbiological Antibiotic Assay I. Factors Influencing Variability and Error. Applied Microbiology. 1971, 22(4), 659-665.
Szczerbiec, D., Piechocka, J., Głowacki, R., Torzewska, A. Organic Acids Secreted by Lactobacillus spp. Isolated from Urine and Their Antimicrobial Activity against Uropathogenic Proteus mirabilis. Molecules 2022, 27(17), 5557. doi:10.3390/molecules27175557.
Ambrosio, C.M.S., Ikeda, N.Y., Miano, A.C., Saldaña, E., Moreno, A.M., Stashenko, E., Contreras-Castillo, C.J., Da Gloria, E.M. Unraveling the selective antibacterial activity and chemical composition of citrus essential oils. Sci Rep. 2019, 9(1), 17719. doi:10.1038/s41598-019-54084-3.
Qian, Y., Gui-bing, L., Wen-qiong, W., Cong-Cong, T., Ruixia, G. The mechanism of whey protein and blueberry juice mixed system fermented with Lactobacillus inhibiting Escherichia coli during storage. Sci Rep. 2023, 13(1), 6614. doi:10.1038/s41598-023-33888-4.
Elughi, G.N., Oniha, M.I., Obafemi, Y.D., Akinyosoye, A.D., Ahuekwe, E.F., Akinduti, P.A. Antibacterial Activity of Cell-Free Supernatants of Probiotic Lactobacillus against Bacterial Pathogens Associated with Vaginal Infections. J Pure Appl Microbiol. 2024, 18(1), 451–459. doi:10.22207/JPAM.18.1.28
Yang, K.M., Kim, J.S., Kim, H.S., Kim, Y.Y., Oh, J.K., Jung, H.W., Park, D.S., Bae, K.H. Lactobacillus reuteri AN417 cell-free culture supernatant as a novel antibacterial agent targeting oral pathogenic bacteria. Sci Rep. 2021, 11(1), 1631. doi:10.1038/s41598-020-80921-x
Chen, C.C., Lai, C.C., Huang, H.L., Huang, W.Y., Toh, H.S., Weng, T.C., Chuang, Y.C., Lu, Y.C., Tang, H.J. Anti-microbial activity of lactobacillus speciesagainst carbapenem-resistant enterobacteriaceae. Front Microbiol. 2019, 10, 789. doi:10.3389/fmicb.2019.00789
Mohapatra, A.R., Lakshmanan, D., Jeevaratnam, K. Anti-biofilm activity of new low molecular weight compounds pro-duced by Lactiplantibacillus plantarum SJ33 against Klebsiella pneumoniae. bioRxiv 2024, 2024, 12. doi:10.1101/2024.12.03.626605
Lee, Y.B., Byun, E.J., Kim, H.S. Potential role of the microbiome in acne: A comprehensive review. Journal of Clinical Medicine 2019, 8(7), 987. doi:10.3390/jcm8070987 PubMed PMID: 31284694.
Rušanac, A., Škibola, Z., Matijašić, M., Čipčić Paljetak, H., Perić, M. Microbiome-Based Products: Therapeutic Potential for Inflammatory Skin Diseases. International Journal of Molecular Sciences 2025, 26(14), 6745. doi:10.3390/ijms26146745
Habeebuddin, M., Karnati, R.K., Shiroorkar, P.N., Nagaraja, S., Asdaq, S.M.B., Anwer, M.K., et al. Topical Probiotics: More Than a Skin Deep. Pharmaceutics 2022, 14(3), 557. doi:10.3390/pharmaceutics14030557
Dapkevicius, I., Romualdo, V., Marques, A.C., Lopes, C.M., Amaral, M.H. Acne vulgaris Topical Therapies: Application of Probiotics as a New Prevention Strategy. Cosmetics 2023, 10(3), 77. doi:10.3390/cosmetics10030077
Thiviya, P., Gamage, A., Kapilan, R., Merah, O., Madhujith, T. Single Cell Protein Production Using Different Fruit Waste: A Review. Separations 2022, 9(7), 178. doi:10.3390/separations9070178
Krzyżostan, M., Wawrzyńczak, A., Nowak, I. Use of Waste from the Food Industry and Applications of the Fermentation Process to Create Sustainable Cosmetic Products: A Review. Sustainability 2024, 16(7), 2757. doi:10.3390/su16072757
Shah, U., Hajoori, M. Production of Single Cell Protein from Fruit Waste. Int J Res Appl Sci Eng Technol. 2022, 10(8), 1311–1317. doi:10.22214/ijraset.2022.46369
Xavier, J.R., Nallamuthu, I., Chauhan, O.P. Optimisation of lactic acid production using cost effective agro residue for food applications. Sustainable Food Technology 2024, 2(3), 741-749. doi:10.1039/d300213f
Costa, S., Summa, D., Radice, M., Vertuani, S., Manfredini, S., Tamburini, E. Lactic acid production by Lactobacillus casei using a sequence of seasonally available fruit wastes as sustainable carbon sources. Front Bioeng Biotechnol. 2024, 12, 147278. doi:10.3389/fbioe.2024.1447278
Bulgari, D., Gobbi, E., Cortesi, P., Peron, G. Bioconversion of Food and Green Waste into Valuable Compounds Using Solid-State Fermentation in Nonsterile Conditions. Plants 2024, 13(24), 13243494. doi:10.3390/plants13243494
Pandey, V.K., Shafi, Z., Tripathi, A., Singh, G., Singh, R., Rustagi, S. Production of biodegradable food packaging from mango peel via enzymatic hydrolysis and polyhydroxyalkanoates synthesis: A review on microbial intervention. Current Research in Microbial Sciences 2024, 7, 100292. doi:10.1016/j.crmicr.2024.100292
Aiello, F., Restuccia, D., Spizzirri, U.G., Carullo, G., Leporini, M., Loizzo, M.R. Improving kefir bioactive properties by functional enrichment with plant and agro-food waste extracts. Fermentation 2020, 6(3), 8. doi:10.3390/FERMENTATION6030083
Makut, M.D., Emelogu, N.J., Ekeleme, I.K., Owuna, J.E., Alfa, F.U. Antimicrobial Activity of Lactic Acid Bacteria Isolated From Locally Fermented Cow Milk Products Sold in Keffi, Nigeria on Clinical Bacteria. GSC Advanced Research and Re-view 2021, 8(2), 78–84. doi:10.30574/gscarr.2021.8.2.0165
Tsuda, H. Production of reuterin by Lactobacillus coryniformis and its antimicrobial activities. Journal of Dairy Research 2023, 90(3), 312–7. doi:10.1017/s002202992300047x
Bazireh, H., Shariati, P., Jamalkandi, S.A., Ahmadi, A., Boroumand, M.A. Isolation of Novel Probiotic Lactobacillus and Enterococcus Strains rom Human Salivary and Fecal Sources. Front Microbiol. 2020, 11, 597946. doi:10.3389/fmicb.2020.597946
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Copyright (c) 2026 M. Arifuddin, Islamudin Ahmad, Arsyik Ibrahim, Baso Didik Hikmawan, Hifdzur Rashif Rija'i, Riki Riki, Erwin Samsul, Iswahyudi Iswahyudi, Arfina Sukmawati Arifin, Arfiani Arifin, Rezti Amanda, Laode Rijai (Author)

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