In Vitro Free Radical Scavenging Activity of Lagerstroemia speciosa PERS leaves on 1,1-diphenyl-2-picrylhydrazyl

Authors

  • Gita Anna Rezqiyana Fakultas Farmasi Universitas Mulawarman Author
  • Laode Rijai Fakultas Farmasi Universitas Mulawarman Author

DOI:

https://doi.org/10.8406/jpns.v1i1.2534

Keywords:

Antioxidant activity, Free Radical Scavenging Activity, Lagerstroemia speciosa Pers, 1,1-difenil-2-pikrilhidrazil (DPPH)

Abstract

This study aims to determine the yields and free radical scavenging activity based on IC50 value of the extract and the fraction of Lagerstroemia speciosa Pers leaves. The leaves of L. speciosa were macerated using 96% methanol and then practioned using different kinds of solvent based on its polarity including n-hexane, ethyl acetate, and n-butanol. The result showed that the yield of methanol extract obtained from L. speciosa levaes was 5.45% of dried samples obtained, the fraction of n-hexane 19.12%, the fraction of ethyl acetate 4,7%, and the fraction of n-butanol 1.93%. Testing of antioxidant activity is performed with the 1,1-Diphenyl-2- Picryl Hydrazyl (DPPH) by spectrophotometry UV-Visible. Absorbanse measurement was conducted with spectrophotometry at the wave lenght of 516 nm. Data was analyzed with linear regresion to obtain value of IC50. The result of research showed that the extract and fractions of L. speciosa leaves have activity as an antioxidant with the IC50 value of methanolic extract L. speciosa leaves was 12.5 ppm, n-hexane fraction was 18.75 ppm, ethyl acetate fraction was 21.87 ppm, n-butanol was 8.37 ppm.

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Published

12-04-2024

Data Availability Statement

data include in manuscript

How to Cite

Rezqiyana, G. A., & Rijai, L. (2024). In Vitro Free Radical Scavenging Activity of Lagerstroemia speciosa PERS leaves on 1,1-diphenyl-2-picrylhydrazyl. Journal of Pharmaceuticals and Natural Sciences, 1(1), 25-34. https://doi.org/10.8406/jpns.v1i1.2534